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To verify that the SAT1 disruption cassettes were successfully excised from GDH2/gdh2 and GDH3/gdh3 strains, genomic PCR was used with primers C1 and C4 to amplify the region between the upper end of 5′ flank sequences to the lower end of 3′ flank sequences to make sure that both alleles of each gene were removed (Supplementary Fig