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Animal experiment Twelve-week-old male C57BL/6J mice (Long-Life Biotechnology, Liaoning, China) were maintained in filter-top cages in a temperature-controlled room (22°C–24°C) with a 12-h light/12-h dark diurnal cycle and given food and water ad libitum in accordance with the regulations designated and approved by the Northeast Agricultural University Committee on Animal Resources (UCAR), which adheres to FDA and NIH animal care guidelines and reviews all animal protocols prior to approval (NEAUEC20220231)

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This has the net effect of preventing intestinal cholesterol absorption