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For M2 macrophages, some subtypes of M2 macrophages (M2a, M2c) produce anti-inflammatory factors such as IL10 and TGFβ, while some subtype (M2b) secrete TNFα, IL1β, IL6 and IL10 (77), and intraperitoneal injection of IL1β could induce accumulation of the end-products of lipid peroxidation in all structures of the brain in rats (78)

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Mixing the sample or standard with the supplied detection reagent and GSH and incubating at room temperature for 30 minutes yields a fluorescent product which is read at 460 nm in a fluorescent plate reader with excitation at 390 nm