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They can, however, also be formed as a result of stimulation by environmental agitations, such as nutritional deficiency, drought, salinity, solar heat and radiation, exposure to heavy metals, and exposure to pesticides and herbicides [25], hence leading to oxidative distress, i.e., overexposure to oxidants
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Upon reaching these criteria or at the scheduled time for tissue collection, all animals were euthanized by an intraperitoneal (i.p.) injection of an overdose of sodium pentobarbital (150 mg/kg)