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If you added 2.5mL instead of 2.0mL to a 5mg vial, concentration is now 2mg/mL (2000mcg/mL) instead of 2.5mg/mL

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Astaxanthin is a red-orange carotenoid (called a xanthophyll) concentrated in microalgae, salmon, trout, krill, and shrimp

The spin columns are supplied with a resin bed volume of 0.2