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This paper aims to delve into the complexities of LL-37, uncovering its multifaceted nature and potential clinical applications

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Repeated Freeze-Thaw Cycles Each freeze-thaw cycle stresses the peptide structure: - Ice crystal formation during freezing can cause mechanical disruption of peptide conformation - Thawing concentrates solutes at ice-liquid interfaces, creating transient high-concentration micro-environments that favor aggregation - Temperature transitions stress the disulfide bridge Limit freeze-thaw cycles to three or fewer