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GPX4 genetic silencing in vitro The lentiviral plasmids, either non-targeted vector (pLKO.1-shNT) or pLKO.1-shGPX4 plasmid DNAs (Sigma-Aldrich) were transfected along with packaging and envelop plasmids psPAX2 and pMD2.G into HEK293T cells

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Etiological Factors in Parkinson's Disease Parkinson's disease is a multifactorial neurodegenerative disorder arising from a complex interplay of genetic predispositions, environmental influences, aging, and dietary factors (Figure 3)