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It is worth noting that the target BODIPY 318 can be prepared by another route (see Scheme 172 below).[319] In 2023, the same research group [319] presented an optimized route to the synthesis of helical chiral N,N,O,C-BODIPY 319 from the corresponding dipyrromethenes (Scheme 172)

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For kinetic measurements (wild-type and mutants), we used 3–750 µM of substrate and 0.1 µg of protein