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[DOI] [PMC free article] [PubMed] [Google Scholar] 144.Ma S., Mangala L.S., Hu W., et al

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Flavor:Death by Chocolate

Samples for TMAO levels’ quantification were prepared as follows: an amount of 25 μL of each sample was collected, and 3 μL of D9-TMAO (5 μM concentration) and 122 μL of a cold acetonitrile:methanol mixture (50:50, v/v) were added for protein precipitation