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Cellular distribution and regulation are shown in Figure 1
laboratory preparation water
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Western blot analysis The protein lysates were harvested from the cells using the radioimmunoprecipitation assay lysis buffer containing 50 mM of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (pH 7.5), 150 mM of sodium chloride, 10 mM of ethylenediaminetetraacetic acid, 10% glycerol, 1% Triton X-100, 1 mM of sodium orthovanadate, 0.1 mM of sodium molybdate, and 1 mM of phenylmethylsulphonyl fluoride, according to a procedure described previously.24 Protein content was estimated by using the bicinchoninic acid method with bovine serum albumin as standards
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