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Cell Culture and in vitro Model of Hemorrhagic Toxicity Astrocytes and 293T cells (Chinese Academy of Sciences, Shanghai) were cultured in dulbecco’s modified eagle medium (DMEM) (Gibco, UA) added 10% fetal bovine serum (Gibco, Australia), following which they were incubated at 100% humidity in an environment containing 5% CO 2

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We had been making mathematical models of different parts of OCM since 2002, so it was relatively straightforward to put in the effects of estradiol and testosterone into the models and find out the male-female differences in OCM, and this led to Project 1