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Reaction mixture II for each sample was collected as follows: 1160 μl of 0.1-M Tris-HCl buffer with 0.34-mM EDTA (pH 8.5) were sequentially added to 108 μl of each supernatant containing residual GSH, and then at the same intervals, 9 μl of a solution of 5,5’-dithiobis(2-nitrobenzoic acid) or Ellman’s reagent (4 mg/ml of absolute methanol) was added to each sample (in the same sequence of samples)

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A single chlamydial protein reshapes the plasma membrane and serves as recruiting platform for central endocytic effector proteins